Study of the antimicrobial effect of ultraviolet irradiation with a wavelength of 365nm and low-energy laser irradiation of 630-670nm using photosensitizers on the suspension of the culture of Candida albicans and Fusarium spp. (in vitro).

Authors

  • Людмила Тройченко Институт глазных болезней и тканевой терапии им В.П.Филатова
  • Galyna Drozhzhyna ДУ «Інститут очних хвороб і тканинної терапії ім В.П.Філатова НАМН України»
  • Alla Moloda ДУ «Інститут очних хвороб і тканинної терапії ім В.П.Філатова НАМН України»
  • Liudmula Dolenko

Keywords:

ultraviolet irradiation, low-energy laser irradiation, antimicrobial action, Candida albicans, Fusarium spp.

Abstract

Actuality. Infectious corneal ulcers and keratitis, according to the WHO, are one of the leading causes of vision loss and blindness in the world. Despite the large number of antimicrobial drugs, it remains important to find new treatments that can provide rapid and complete microbicidal and microbostatic activity with minimal toxic effects on the body to preserve vision in severe infectious diseases of the cornea.

Objective: To evaluate the antimicrobial effect of the combined method of corneal collagen crosslinking with the inclusion of 0.1% riboflavin and photodynamic therapy in an experiment (in vitro) on cultures of Candida albicans and Fusarium spp.

Materials and methods. The antimicrobial activity of the drugs was studied by the standard disco-diffusion method (MB9.95-143-2007) using Mueller Hinton's nutrient medium.

Methods of exposure: Photodynamic therapy (PDT) - on the surface of a sterile disk, which was on the surface of the nutrient medium with test strains of Candida albicans and Fusarium spp. instilled 0.1% methylene blue, then for 3 minutes was irradiated disk with low-energy laser radiation of 630-670 nm. When performing collagen crosslinking (UVX), 0.1% of riboflavin was instilled, then ultraviolet irradiation of the disk was performed on a UVX 2000 device with a wavelength of 365 nm for 10 minutes.

Results. According to the analysis of growth retardation zones, Candida albicans was affected by both PDT and UVX. A significant difference of 6.3 mm in the growth retardation zone was observed when using the combined PDT +UVX + fluconazole method. On Fusarium spp. had the effect of both the combined PDT + UVX method, and in combination with intraconazole. The significant difference in growth retardation zones was 4.2mm and 7.8mm, respectively.

Conclusions. It was found that the proposed combination of photodynamic therapy with crosslinking of corneal collagen has an antimicrobial effect on Candida albicans and Fusarium spp in an in vitro experiment. The results obtained in the experiment showed the prospects of the proposed combination treatment, which is confirmed by the delay of the growth zone of Candida albicans and Fusarium spp. and require further study in ophthalmology for the treatment of local infections.

Published

2021-11-10

How to Cite

1.
Тройченко Л, Drozhzhyna G, Moloda A, Dolenko L. Study of the antimicrobial effect of ultraviolet irradiation with a wavelength of 365nm and low-energy laser irradiation of 630-670nm using photosensitizers on the suspension of the culture of Candida albicans and Fusarium spp. (in vitro). J.ophthalmol.(Ukraine) [Internet]. 2021 Nov. 10 [cited 2023 Feb. 5];500(3). Available from: https://ojs.ozhurnal.com/index.php/files/article/view/22